FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_31013.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_31013.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18161376
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC45600.017.329433145
TACACTG151300.017.109255
TATACTG201750.015.9577295
GTATAGG71350.014.94131
CTACACT111800.014.5280694
GTGTAAG111750.014.4717931
CTTACAC115850.014.332623
GTATAGA131750.014.3115861
GTACTAG55650.014.0741981
GTGTAGG81400.013.9875051
GTGTTAG132000.013.5153171
GTCCTAC53700.013.5048821
CTAGTAC54000.013.1588653
TATACAG169650.013.121585
CTATACT127550.013.0752214
GTATTAG123400.013.0467661
TACACAG181750.013.0459295
CCTACAC83050.013.0086663
GTATAAG119200.012.8372271
TCTCCGC196400.012.624302145
Produced by FastQC (version 0.11.5)