FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_31013.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_31013.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17839283
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG41050.020.4864145
TATACTG213700.015.5063375
GTGTAGG81200.014.6469991
CTATACT130800.014.1915484
TCTATAC129750.013.9149293
GTACTAG59200.013.7200981
TACACTG157250.013.648935
CTACACT111900.013.2837694
CTCCGAC36200.013.21771145
TACACAG188100.013.1450775
CTTACAC111400.013.0827753
GTATAGA128150.013.0723681
GTCCTAG77400.012.9300151
GTCTCGC57800.012.9232051
GTATTAG129600.012.8141961
CCTATAC74850.012.7870733
TGCACTG168850.012.7112495
TCTACAC116950.012.58591653
GTGTAAG113100.012.5676571
CCTACAC83050.012.3976033
Produced by FastQC (version 0.11.5)