FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30903.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30903.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16515230
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG125550.015.709085
GTATAGG67100.015.6711871
TATACTG171150.014.9977075
GTGTAGG79950.014.6037311
AGACGGC216800.014.546246145
GTGTAAG95350.014.3746571
TACACCG38750.014.2213375
ATGCCGG155950.014.085693145
TACACAG149150.013.9040495
CTATACT115500.013.8741014
TATACAG151650.013.3879535
CTACACT99650.013.2430714
GTCCTAC52700.013.0728241
CTTACAC98850.012.836623
TCTATAC117600.012.7013313
TAGACTG97700.012.6911245
CTCCGAC52250.012.626299145
TAATACT187600.012.4843024
ACGGACG46500.012.472629145
GTATAGA118750.012.4581331
Produced by FastQC (version 0.11.5)