FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30900.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30900.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472112
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG77500.015.6232935
TATACCG29050.014.9748675
TATACTG108350.014.8553155
CTATACT80000.014.5912214
GTGTAGA79050.014.4025921
TATACAG101950.013.5832595
CTACACT64950.013.5070964
TACACAG89250.013.2415015
TAGCGCG33400.013.241116145
TACACCG36700.013.2362995
CCTATAC49700.012.9834283
TCTATAC84800.012.91031553
TCTCCGC86300.012.853515145
GTATAAG82400.012.8489761
GTATAGG49000.012.5795681
TAGGACT55650.012.37696554
CTTACAC63600.012.3118253
GTATTAG83200.012.2896251
CTAATAC89100.012.2058863
TAGACAG94000.012.1095965
Produced by FastQC (version 0.11.5)