FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30890.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30890.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22390347
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG81250.018.47007145
TATACTG240950.016.0395325
TACACTG183350.014.9882075
CTATACT168650.014.9616484
GTCCTAG94350.014.6808681
CTCCGAC55550.014.616909145
TATACCG28600.014.4510775
TCTATAC162550.014.0509993
GTATAGA157000.013.8574041
GTATTAG163850.013.8534571
TCTCCGC233050.013.6564245145
GTATAGG91650.013.1351761
ATGCCGG265750.013.067239145
GTGTAGG102000.013.0110151
CTACACT141850.012.9323594
TAGACTG145350.012.9204045
CCTACAC105850.012.8780653
GTATAAG156950.012.7990781
CCTATAC89950.012.736193
CTAGTAC71850.012.5134933
Produced by FastQC (version 0.11.5)