FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30887.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30887.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001777
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG88550.015.80151145
TATACTG231300.015.078185
CTCCGAC39900.014.899468145
TCTCCGC149750.014.3786955145
GTGTAGG96550.013.5950331
GTATAGA145650.013.5927111
TACACTG186700.013.2819325
GTATAGG82050.013.1692681
GTATTAG147700.013.0112591
CTACACT133600.012.8623814
TCTATAC147700.012.8124523
GTGTAGA126800.012.6394151
CTATACT156000.012.5492754
ATACTGT298700.012.4526756
TATACCG22750.012.4297585
CTTACAC128650.012.3989563
CCTACAC96000.012.3108663
TACACAG222100.012.0137535
CCTATAC84800.011.8847813
TAGACTG146650.011.86611755
Produced by FastQC (version 0.11.5)