FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30886.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30886.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19450310
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG164950.015.9566225
TATACTG227700.015.6671055
CTACACT121000.014.9210164
TACACCG30100.014.6943385
GTATTAG142850.014.5193911
CTATACT148000.014.4035664
TCTATAC144400.014.2602693
TCTCCGC150550.014.109369145
GTGTAGG90250.013.981851
TATACAG191900.013.8290645
CTCCGAC42200.013.743531145
CCTACAC89000.013.6866093
TATACCG23900.013.6521575
TAGACTG132800.013.4314755
ATACTGT296950.013.3076036
GTGTAGA127600.013.299271
GTATAAG134650.013.2492511
GTCCTAG84050.013.11500551
GTATAGG78100.012.9998871
GTATAGA144200.012.9249961
Produced by FastQC (version 0.11.5)