FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30886.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30886.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20444581
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG107150.020.906878145
TATACTG240500.016.6724265
CTCCGAC46150.016.49455145
TACACTG172750.015.9077915
GTATAGG85500.014.5041421
CTATACT157450.013.999734
GCTAGAC89300.013.8844643
GTATAGA151900.013.7975541
CTACACT130600.013.4357194
TAGACAG259750.013.3990625
TATACAG195150.013.3015795
GTATAAG137250.013.1039241
GTATTAG149250.013.0221441
TCTCCGC184500.012.967028145
GTCTCGC65450.012.9639771
GTGTAGA127050.012.9001811
GTCTAGG92900.012.8804261
GTGTAAG130550.012.7765321
GACTCCG80950.012.7170667
TCTATAC150450.012.6750023
Produced by FastQC (version 0.11.5)