FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30849.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30849.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19167437
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG89850.016.7047085
TACACCG133750.015.7755985
TATACTG122200.014.7152325
GTGTAGG105950.014.6475561
TATACCG75500.013.8293635
GTATAGA141250.013.8107151
CTATACT116000.013.6265254
CTACACT90650.013.5977714
GTATAGG87000.013.5035541
CTTACAC94500.013.2736963
TAGACTG64050.013.1318365
TATACAG140900.013.1224775
GTGTAAG95700.012.9579561
GTATAAG138650.012.9190151
TACACAG103100.012.8700145
CTACCCT78250.012.7873744
GTATTAG163650.012.6293551
CTACACC184800.012.4770464
TTATACT203100.012.4595434
TCTACAC138600.012.4506283
Produced by FastQC (version 0.11.5)