FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30848.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30848.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17630959
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG104700.016.0667045
TATACTG154250.015.9352915
TACACCG88000.015.4903285
CTATACT129800.015.3618474
TACACAG124650.014.4841345
TATACAG169150.013.9743315
CCTACAC104100.013.7211693
GTGTAGG97100.013.3684051
CTACACT99350.013.35576254
TCTATAC154600.012.9910983
TATACCG70600.012.9405085
GTCCTAG38550.012.6036481
TAGACTG75350.012.5096625
GTATAAG152500.012.4588311
CTAGACT46100.012.4254364
GTATAGG94750.012.322321
GTCCTAC70400.012.2580151
CTACCCT74150.011.9298284
GTGTAGA138600.011.9293671
TTACACT145900.011.927284
Produced by FastQC (version 0.11.5)