FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30844.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30844.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15152899
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG106750.018.7474945
CTACACT89500.015.8794364
TATACTG151200.015.0584365
GTATAGA112750.014.3434421
TACACCG43300.014.2341995
CCTACAC74050.013.8065313
TATACAG144600.013.73992355
TAGCGCG57050.013.724244145
GTGTAGG72800.013.6475361
TAGACTG85150.013.6250275
TATACCG34600.013.621955
TGCACTG119750.013.5635865
GTATAGG64800.013.5417711
CTAGGAC51300.013.427553
TCTACAC108200.013.402733
GTATTAG120600.013.2294091
CTATACT108000.013.1593474
TAGACAG152350.013.0409775
TCTATAC115300.013.017623
GTGTAGA100300.013.0147751
Produced by FastQC (version 0.11.5)