FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30830.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30830.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15134720
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC113650.015.437422145
TATACTG162850.015.4048465
GTCCTAG62100.015.0640491
TACACAG149900.014.7041855
CTACACT94550.014.3399984
GTGTAAG97450.014.1388911
ATGCCGG117500.014.067788145
TACACTG121700.013.9410115
GTGTACG25300.013.7582861
CTCCGAC36900.013.753097145
GTATTAG114850.013.6384941
GTGTAGA92900.013.42631
CCTACAC69100.013.3257553
TATACCG23700.013.1549475
TCTATAC110250.013.152783
CGTACAC24150.012.90973953
CTACCCT78450.012.8466784
GTACTAG50050.012.7503541
TCTACAC100550.012.6189183
GTGTTAG103450.012.5477591
Produced by FastQC (version 0.11.5)