FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30786.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30786.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15631397
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG183850.016.1299935
TACACCG23950.016.0451895
GACCGGC118750.015.872987145
GTATAGA116700.014.4792841
CTATACT117000.014.1913184
TACACAG158100.013.9875765
TCTCCGC136800.013.937618145
CTCCGAC36000.013.895224145
CTTACAC99000.013.7686143
TACACTG130000.013.664615
GTGTAGG73600.013.49911
CCTATAC65350.013.3138363
GTCCTAG66350.013.0067281
CTAGACT82800.012.9599864
GTATAAG105050.012.9094461
GTGTAGA97100.012.8460991
CTACACT101050.012.8436914
TCTATAC115800.012.772893
GTACTAG48500.012.7098151
CTAACAC120600.012.6853583
Produced by FastQC (version 0.11.5)