FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30785.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30785.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20296564
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG194800.015.78185
TACACTG139850.015.6575615
CTACACT110100.015.3443034
ATGCCGG159450.014.867561145
TATACCG38150.014.2543145
GTATAAG134000.013.8547031
CTATACT137850.013.8333554
TACACCG63950.013.8324445
GTATAGG84300.013.764311
TAGACAG195350.013.6959645
TAGACTG109350.013.1951375
GTACTAG57550.013.1053931
CGAGACA91000.012.9856205145
GTGTAGA131800.012.9304761
GTGTAAG113600.012.8954081
CTAGTAC61500.012.8506673
GTATAGA138550.012.8239421
TCTACAC136000.012.7951873
CCTATAC87550.012.5881453
TCTCCGC253250.012.509746145
Produced by FastQC (version 0.11.5)