FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_30783.TR5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_30783.TR5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14789052
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT84100.016.3809874
TATACTG140950.016.2556535
TATACCG30350.016.006585
CTATACT105000.015.8825774
GTATAGG60800.015.5063251
TACACTG99000.015.3071225
GTGTAGG69650.015.3061271
TACACCG43550.015.1508035
ATGCCGG163000.013.92151145
GACCGGC164200.013.68731145
GTATTAG109800.013.4740111
CCTATAC65150.013.4663223
TAGTACT78800.013.4341144
GTGCTAG59500.013.0417511
TACACAG121900.013.0263535
TCTATAC105700.012.7589813
TAGGACA81300.012.5750884
GTCCTAC50800.012.5628471
GTGTAGA93000.012.4768841
GTGTTAG88150.012.4229211
Produced by FastQC (version 0.11.5)