FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26070794
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10436894.003288123867651No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2801571.07460095001326No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508300.19496912905682887No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAA442450.16971098003382637No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAC439740.1686715026784378No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAT386940.148418954942454No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAA372640.14293388993062506No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAC370680.14218209081012262No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAG335400.1286497066410789No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAT329930.12655157338130937No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAG285040.10933307209592465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC1358200.043.5972941
ATAGCTC1368700.043.4125333
CATAGCT1364250.043.3395652
TAGCTCT1367650.043.2145464
GTCCGAC366600.042.7023114
TAATGCG259250.042.564055
TGTCCGA367750.042.53327613
CGACAAT377600.042.50922817
TTGTCCG369700.042.24386212
CCGACAA381750.042.1798416
AGTTGTA551750.042.1757667
GTTGTAC546050.042.090538
TCCGACA376300.042.01352715
TTGTACC539150.042.009119
ACCCGTG227800.041.92431314
TCACCTA305400.041.909139
CAGTCGA198100.041.6448863
AGTCGAT198500.041.610254
AACGCTT259800.041.50410513
TAGCGCA228750.041.45165621