FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15751144
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2424231.5390818597049205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG853720.5420050759487691No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473560.30065117809855585No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT162920.10343375693854363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT300950.038.0668331
AGCAGTG331100.034.5431942
ATCAACG369500.030.81240511
TCAACGC370950.030.66891712
CAACGCA373850.030.4487713
GTATCAA376950.030.1520429
TGGTATC377700.030.1141387
AACGCAG382200.029.85254714
TATCAAC382000.029.82813610
GGTATCA383250.029.6677868
ACGCAGA384800.029.59796115
GTGGTAT385300.029.5482236
AGAGTAC384500.029.46917219
CAGTGGT389000.029.3215964
AGTGGTA390400.029.1613865
CGCAGAG395700.028.88803116
CAGAGTA396550.028.70885518
GCAGAGT401250.028.39496417
GAGTACT400100.028.24245620
GTACTTT399100.028.1285322
Produced by FastQC (version 0.11.5)