FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13627857
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2223941.6319073497762706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG694170.5093757587858458No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT429090.3148624174732682No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT151170.11092719860503379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT272400.038.6565671
AGCAGTG303050.034.7288252
ATCAACG338500.030.97592411
TCAACGC341350.030.67895912
CAACGCA343250.030.56694813
TGGTATC343700.030.5456877
GTATCAA344850.030.4133729
TATCAAC348000.030.15649810
GGTATCA349500.030.046518
AACGCAG350900.029.94390714
GTGGTAT351400.029.8940496
ACGCAGA351450.029.83894215
CAGTGGT353000.029.8133414
AGAGTAC351500.029.78847119
AGTGGTA354450.029.7107645
CGCAGAG361800.029.03986216
CAGAGTA361750.029.03715518
GCAGAGT363850.028.8277617
GTACTTT364450.028.53377722
GAGTACT366600.028.4590620
Produced by FastQC (version 0.11.5)