FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15268198
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2366601.550019196764412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG774040.5069622492451303No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT461260.3021050683256793No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT162540.10645657070991613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT294450.038.336631
AGCAGTG330550.034.2788282
ATCAACG368800.030.4717511
TCAACGC371650.030.24449712
CAACGCA374400.030.0046213
GTATCAA376800.029.8546779
TGGTATC377900.029.7837817
TATCAAC377600.029.77996410
GGTATCA382250.029.4405338
ACGCAGA383550.029.3266815
AACGCAG384250.029.29788814
CAGTGGT385500.029.296184
GTGGTAT385400.029.2829636
AGTGGTA387350.029.1575035
AGAGTAC385150.029.0817319
CGCAGAG394750.028.53873416
CAGAGTA396200.028.35024318
GCAGAGT397100.028.28617717
GAGTACT395600.028.14065420
GTACTTT397900.027.93128222
Produced by FastQC (version 0.11.5)