FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14873656
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2417381.6252762602550441No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG781730.5255802608316341No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT467560.3143544532695929No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163600.10999313148024938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT298250.038.558421
AGCAGTG332100.034.5977132
ATCAACG370550.030.86133211
TCAACGC372450.030.68129712
CAACGCA374100.030.53359413
TGGTATC375200.030.4264837
GTATCAA377850.030.2554849
TATCAAC379250.030.1898110
GGTATCA381450.029.9467648
CAGTGGT385350.029.7701234
ACGCAGA384600.029.73774115
AACGCAG385000.029.73742114
GTGGTAT385050.029.7213336
AGAGTAC384000.029.68460319
AGTGGTA388650.029.5468435
CAGAGTA395950.028.9187418
CGCAGAG396650.028.89524816
GAGTACT396300.028.59653520
GCAGAGT400050.028.57874717
GTACTTT397550.028.3826722
Produced by FastQC (version 0.11.5)