FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12630092
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3676162.9106359636968597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG838810.664136096554166No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT704120.5574939596639518No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT233210.18464631928255154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT437500.040.7844051
AGCAGTG472100.037.753532
CAGTGGT556500.031.9619064
ATCAACG563300.031.5097611
AGTGGTA569050.031.308645
TGGTATC567350.031.2957217
TCAACGC567600.031.22840312
GTATCAA569250.031.1890059
CAACGCA569500.031.1354413
TATCAAC571850.031.07004210
GGTATCA571800.031.0614628
AACGCAG573750.031.01219414
ACGCAGA573900.030.9791215
GCAGTGG581850.030.7023623
AGAGTAC576350.030.69204519
GTGGTAT581800.030.5743226
CGCAGAG582700.030.5184616
CAGAGTA586100.030.29952218
GCAGAGT587950.030.14840917
GAGTACT586550.030.00744220
Produced by FastQC (version 0.11.5)