FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17777320
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5016782.8220114167939823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1193960.6716197942097009No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT980680.5516467049026512No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314660.1770008077708001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT591250.040.7344321
AGCAGTG638300.037.684182
CAGTGGT757150.031.7648184
ATCAACG764300.031.40830411
TGGTATC769850.031.2127917
TCAACGC770300.031.11812812
GTATCAA772800.031.0951279
CAACGCA772550.031.04432713
AGTGGTA778550.030.9411335
TATCAAC777400.030.91061410
GGTATCA777950.030.8920218
AACGCAG779600.030.81678614
ACGCAGA779550.030.77777115
AGAGTAC781750.030.59534319
GTGGTAT789000.030.4765836
CGCAGAG790750.030.3747216
GCAGTGG797650.030.2790743
CAGAGTA797150.030.07798418
GCAGAGT799900.029.96124617
GAGTACT797100.029.87048720
Produced by FastQC (version 0.11.5)