FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505208
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4803632.9103722897645397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1114320.6751323582229318No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT935200.5666090363720349No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT307820.18649870998293386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT575400.040.572621
AGCAGTG619550.037.6698952
CAGTGGT734250.031.6910784
ATCAACG740550.031.3721811
TCAACGC745750.031.14790712
TGGTATC746000.031.1329147
GTATCAA748050.031.0258529
CAACGCA749200.031.0131913
TATCAAC751300.030.9386910
GGTATCA751900.030.8959718
AGTGGTA754250.030.8897885
AACGCAG757000.030.7511214
ACGCAGA757500.030.70262715
AGAGTAC756250.030.52966719
GTGGTAT767400.030.3382686
GCAGTGG771550.030.2879683
CGCAGAG769350.030.26967616
CAGAGTA772050.030.03144518
GCAGAGT774000.029.95917117
GAGTACT767900.029.9167820
Produced by FastQC (version 0.11.5)