FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16185058
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4743472.9307710852812514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1068490.6601706339266749No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT911480.563161404796943No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT298890.18467032988080734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT555450.040.7467581
AGCAGTG600950.037.665292
CAGTGGT714750.031.6142734
ATCAACG724900.031.18018711
TGGTATC729800.030.949547
TCAACGC730200.030.9272312
GTATCAA732850.030.8611039
CAACGCA732950.030.82010513
TATCAAC736200.030.7381310
AGTGGTA736100.030.7223765
GGTATCA736900.030.6972718
AACGCAG738900.030.59258314
ACGCAGA741750.030.4531415
AGAGTAC741300.030.31168719
GTGGTAT747700.030.2140256
GCAGTGG750650.030.2108923
CGCAGAG753350.029.98330116
CAGAGTA756250.029.86268618
GCAGAGT759100.029.73644417
GTACTTT750100.029.68622622
Produced by FastQC (version 0.11.5)