FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15552977
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1940701.2477996977684722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG696410.44776636652905744No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368040.23663636871577706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT250550.036.952781
AGCAGTG281550.032.878832
ATCAACG299700.030.63874211
TCAACGC302600.030.30875612
CAACGCA304600.030.1094613
GTATCAA311000.029.5755089
TGGTATC311900.029.5311667
TATCAAC312350.029.49680310
AACGCAG314600.029.2359414
AGAGTAC313800.029.20125419
GGTATCA316800.029.110168
ACGCAGA315900.029.06509415
GTGGTAT319400.028.9263576
AGTGGTA324450.028.5024225
CAGTGGT325350.028.409054
CGCAGAG328700.028.10033816
CAGAGTA329300.027.94160318
GAGTACT328250.027.8080920
GCAGAGT331650.027.72433717
GTACTTT332500.027.39821822
Produced by FastQC (version 0.11.5)