FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15895732
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1997901.256878261410044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG676830.42579354005213477No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT374440.2355600861916897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT255700.036.8703961
AGCAGTG287000.032.922362
ATCAACG307350.030.72908811
TCAACGC308600.030.60471712
CAACGCA310850.030.39008113
GTATCAA314800.029.9609579
TGGTATC316000.029.7202427
AACGCAG319150.029.65480414
TATCAAC318750.029.60292410
ACGCAGA319200.029.57926415
GGTATCA320700.029.3681228
AGAGTAC321950.029.21495619
GTGGTAT325250.028.9211836
CAGTGGT328400.028.6889554
AGTGGTA329150.028.5979275
CGCAGAG333900.028.36884316
CAGAGTA336700.028.09327718
GCAGAGT336250.028.08559617
GAGTACT335000.027.95795620
GTACTTT336800.027.73406222
Produced by FastQC (version 0.11.5)