FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20022893
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2415541.2063891067090056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG907650.45330612314614077No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT458060.2287681405479218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT315950.036.8022081
AGCAGTG352650.032.96972
ATCAACG382750.030.30364211
TCAACGC385900.030.02799412
CAACGCA387150.029.89140913
GTATCAA391700.029.64089
TATCAAC394550.029.42045410
TGGTATC394600.029.3605947
AACGCAG399050.029.07671414
ACGCAGA398800.029.059815
GGTATCA400600.028.976868
AGAGTAC400650.028.81304419
GTGGTAT405150.028.6437156
CAGTGGT411800.028.1949334
AGTGGTA411700.028.176475
CGCAGAG412950.028.12244616
CAGAGTA418150.027.72460418
GAGTACT414650.027.71225220
GCAGAGT419950.027.5537917
GTACTTT418950.027.27846322
Produced by FastQC (version 0.11.5)