FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_cDNA_1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_cDNA_1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18106704
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2095591.1573558611219357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG794310.43868282156708366No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT404310.2232929858465682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT278900.036.698921
AGCAGTG316150.032.3629652
ATCAACG333200.030.53042611
TCAACGC335500.030.2689112
CAACGCA337100.030.14449313
GTATCAA342650.029.7085119
TGGTATC344750.029.5265667
TATCAAC345450.029.47359510
AACGCAG347950.029.24204614
ACGCAGA347850.029.15395415
GGTATCA349050.029.107138
AGAGTAC349850.028.88418819
GTGGTAT354150.028.8097726
AGTGGTA359550.028.4496565
CAGTGGT358950.028.4226344
CGCAGAG362000.028.06884616
CAGAGTA361900.028.0089518
GCAGAGT367500.027.57070417
GAGTACT365350.027.54374520
GTACTTT366550.027.37973222
Produced by FastQC (version 0.11.5)