FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_BLG_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_BLG_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9908735
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293430.296132654672872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1901050.043.63164511
CAACGCT1902800.043.5824312
AACGCTT1904550.043.57105313
TGTCCTA1414300.043.53912411
GTCCTAC1414100.043.4936912
AGTCTTT826900.043.48385210
GTCTTTG2721300.043.4777411
TGTCCGA3452850.043.47604413
TTGTCCG3456400.043.47426612
GTCAACG890200.043.46693810
TCCGACA3455050.043.43257515
CGCTTGG1904150.043.4310315
TCCTACC1409050.043.42915313
CTGTCCT360500.043.42477410
GTCCGAC3452650.043.4241514
CTCAACG491900.043.4068410
ACGCTTG1908650.043.39357414
CCGACAA3457200.043.3751916
GGTCTTT945750.043.361610
TTGGCTA1907800.043.3588718
Produced by FastQC (version 0.11.5)