FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_BLG_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_BLG_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9179020
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284080.30948837675481694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA1493250.043.62504611
GTCCTAC1493300.043.589712
ATGTCCT371150.043.56400710
TCAACGC1459500.043.558911
TCCTACC1488550.043.55288313
CAACGCT1459350.043.5513212
AACGCTT1461100.043.5337913
CTGTCCT373150.043.51331710
AGTCTTT857950.043.4982710
GTCTTTG2794000.043.4750111
TTGTCCG3035100.043.47023812
TGTCCGA3031400.043.46449713
TCCGACA3032950.043.4290515
GTGTCCT394050.043.42790610
GTCCGAC3031250.043.42407614
CGCTTGG1459650.043.39758315
CCGACAA3034950.043.37650716
ACGCTTG1463400.043.36866414
TCTTTGT2796250.043.3676312
GGTCTTT965450.043.3636510
Produced by FastQC (version 0.11.5)