FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_BLG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_BLG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9903706
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2445532.4693079540123666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC3663850.043.6659111
CAACGCT3665550.043.61466212
GTCAACG1719350.043.58934810
AACGCTT3671100.043.58043313
TGTCCTA1132050.043.53590811
CTCAACG941200.043.5321910
CGCTTGG3662800.043.50910615
ACGCTTG3669900.043.50534414
GTCCTAC1132200.043.50120512
TCCTACC1127400.043.4983913
ATCAACG649500.043.4975510
TTGTCCG2481500.043.47109612
TGTCCGA2480600.043.4507313
AGTCTTT621300.043.4495410
GCTTGGC3665300.043.4239816
TTGGCTA3670250.043.42132618
GTCTTTG2016350.043.412411
TCCGACA2482300.043.4054915
CTTGGCT3673200.043.38907217
CTGTCCT290950.043.3889610
Produced by FastQC (version 0.11.5)