FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_BLG_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_BLG_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11324578
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368180.3251158674521912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA2081450.043.63967511
TCAACGC1971150.043.59899511
GTCCTAC2082750.043.58727612
CAACGCT1971200.043.57018312
TCCTACC2076650.043.55425313
AACGCTT1972100.043.55252513
ATGTCCT510100.043.51322610
CTGTCCT536600.043.5131210
GTGTCCT552350.043.495410
TTGTCCG3609400.043.48591212
GTCTTTG3110600.043.48339511
AGTCTTT966500.043.46744510
TGTCCGA3609150.043.46637313
GTCCGAC3607800.043.4332314
CGCTTGG1970550.043.42630415
TCCGACA3613550.043.42613615
GTCAACG932850.043.41959410
GGTCTTT1079800.043.39795710
TTGGCTA1971950.043.37850618
ACGCTTG1976900.043.37220814
Produced by FastQC (version 0.11.5)