FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_ADT_three.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_ADT_three.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787804
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176670.36900006767194315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT706700.043.8674051
TAACCAG1292000.043.749192
GACAGTC455150.043.7437061
GTTGTAC1288150.043.723678
TTAACCA1292300.043.720971
CCAGTTG1292150.043.7108965
ACCAGTT1291350.043.7080964
CATTCTG708950.043.70612
TACCCCC1273900.043.70008512
GTACCCC1280700.043.68798411
GAGCACC528550.043.6874543
CAGAGCA530950.043.6842961
AGTTGTA1293600.043.6825567
TTGTACC1284300.043.661139
ACAGTCG455150.043.6256182
CAGTTGT1295000.043.616526
TGTACCC1286100.043.5837110
TGTCACC705650.043.5771267
ACCCCCA1430600.043.5715413
TTCTGTC712600.043.5644234
Produced by FastQC (version 0.11.5)