FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_ADT_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_ADT_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115179
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135310.26452642224250605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT743450.043.8792841
TAACCAG1361700.043.796942
ACCAGTT1362850.043.7110864
TACCCCC1347300.043.70020312
CCAGTTG1363500.043.691965
TTAACCA1364000.043.6914861
AGTTGTA1366000.043.6914447
GTACCCC1353500.043.68938411
GTTGTAC1360450.043.686598
GACAGTC499900.043.6690521
GAGCACC551100.043.6666533
ACAGTCG498650.043.666392
CATTCTG746000.043.6610872
TTGTACC1356700.043.6330269
TGTCACC741650.043.621477
CAGTTGT1368250.043.5821846
AACCAGT1368800.043.580883
TCTGTCA747000.043.55735
CAGAGCA552850.043.553021
TGTACCC1358900.043.5485810