FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_ADT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_ADT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449137
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG429320.37498022776738543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT1687350.043.851191
TAACCAG3162400.043.7858162
CATTCTG1690800.043.7662962
TTAACCA3165250.043.7466661
ACCAGTT3164150.043.7209434
GACAGTC1108150.043.720041
TACCCCC3122050.043.69255412
GTTGTAC3157200.043.682928
GTACCCC3138700.043.68270511
CCAGTTG3167350.043.6817175
GAGCACC1279650.043.670723
CAGAGCA1283950.043.6442071
AGTTGTA3173700.043.635577
TTGTACC3150600.043.6050839
TGTCACC1684550.043.6017277
CAGTTGT3175850.043.5869946
ACCCCCA3491300.043.5808313
ACAGTCG1108100.043.57772
AACCAGT3178150.043.568533
GCACCCA1279400.043.539375
Produced by FastQC (version 0.11.5)