FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_ADT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_ADT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17681066
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494980.2799491840593774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT2585350.043.8503271
TAACCAG4820750.043.7908632
GACAGTC1766250.043.7371641
ACCAGTT4823200.043.7184564
CATTCTG2591800.043.7174842
TTAACCA4829750.043.6998371
GTACCCC4788200.043.6885211
TACCCCC4762200.043.6884912
CCAGTTG4827300.043.683975
AGTTGTA4834050.043.6818437
GAGCACC1884850.043.6778033
GTTGTAC4817800.043.6618778
TTGTACC4797450.043.660779
ACAGTCG1766150.043.6151922
CAGAGCA1893900.043.615161
TGTCACC2582950.043.6077657
TCTGTCA2597350.043.5902185
AACCAGT4844200.043.587583
TGTACCC4807600.043.5660610
ACCCCCA5331900.043.56291613