FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_4HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_4HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585105
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347980.7589357277532358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA2505350.043.9265251
CGATAAT2506450.043.8728562
ATAATGC2502200.043.795894
TAATGCG2497850.043.7900665
GATAATG2515000.043.7401733
AGGACCA949550.043.713821
GGACCAT949100.043.6660352
TGCGACC2483250.043.6429758
GCGACCC2479650.043.6264579
CATCCAA955850.043.624656
CCATCCA954650.043.6143885
ATCCAAC957750.043.5781177
TCCAACC954400.043.571588
AATGCGA2505400.043.569626
CGACCCA2485900.043.56887410
GACCATC954900.043.556193
CATGTTA312850.043.554492
ATGCGAC2500650.043.509017
CCAACCC954100.043.5068559
ACCATCC956250.043.503574