FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_4GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_4GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12920928
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAC4760443.6842864537284012No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAA4709673.6449936103660665No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAA4319653.3431422263168713No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAT4249733.2890284660668336No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAC4239823.2813587383197245No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAT3766572.9150924763298733No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAG3650622.82535433987404No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAG3300822.5546307509801154No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAC1973731.5275450803533617No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAA1930171.4938323315477031No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAT1749201.353772732113359No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAG1535091.1880648201119919No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAC1456631.1273416274744352No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAA1434451.1101756777841345No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAC1343961.0401420083758688No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAT1280660.9911517191334864No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAA1226470.9492120070632697No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAC1204230.9319996210798481No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAG1195280.9250728740226708No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAT1164730.9014290614420264No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAA1160860.8984339205357387No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAG1043610.8076896643956224No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAT1016310.7865611510256849No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAG1000930.7746579812224014No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAC939030.7267512054861694No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAC935620.7241120761604739No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAA934840.7235084043499043No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAC933980.7228428174818403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG871910.6748044722484329No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAT861720.6669180418000936No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAA856480.6628626055342155No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAA844780.6538075283756708No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAT820030.6346525574633648No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAT777330.6016053955257703No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAG740290.5729387239058991No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAG737690.5709264845373335No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAA711180.5504093823601525No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAG704890.5455413109646614No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAC691510.535186017598736No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAA647380.5010321240084303No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAC619820.47970238670163634No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAC610820.472736942733525No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAT605840.46888273040450346No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAA571850.44257657035160325No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAT553200.4281426225732393No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAG534640.41377832923455654No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAG527530.40827562849974863No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAT525350.4065884431830283No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAA495090.3831690726858009No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAC493930.3822713043521332No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAG480410.3718076596355927No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAC469100.36305441838233293No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAC459390.35553947827895954No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAA457610.35416186824971085No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCCATATAAGAAA457260.3538909898731732No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAT438790.3395963509741715No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAA417230.3229102429794516No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAT413200.31979127195817514No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCCATATAAGAAA412830.3195049148172639No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAT410950.3180499109661473No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAG408010.31577453260323096No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAC400680.3101015654603137No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAA399320.3090490094829102No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAC392530.3037939689780796No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAA392100.3034611755440476No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAA391610.30308194581689485No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAC373490.28905818529443084No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAG367160.28415915637019257No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAG365030.28251066796440627No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAT361340.27965483593748064No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAA360830.27926012744595435No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAT358110.27715501549114735No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAT348610.2698026024136966No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAG327440.25341833032426153No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAC319210.2470488187845331No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAG312050.24150742113879126No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAG304750.23585767214243436No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAC297730.23042462584730755No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAT297530.23026983820357175No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAA295000.2283117745103138No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAG283580.2194734000529993No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAC276570.21404809314005932No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAA265310.2053335487977334No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAC262540.20318973993199252No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAA262460.20312782487449818No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAT259490.20082922836502146No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAA250310.19372447551754798No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAG245790.19022627476911874No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAG235840.18252558949326242No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAC233110.18041273815626865No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCCATATAAGAAA232720.18011090225098383No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAC226990.17567623625795298No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAT220550.17069207412966003No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAT220390.17056824401467138No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAT219310.16973239073849805No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAT217880.16862565908578703No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAA215980.16715517647029687No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAC209340.1620162266982681No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAG203290.15733390047525997No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAG201530.1559717692103849No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAG199650.1545167653592683No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAC191630.14830978084546248No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAAC190700.14759001830209098No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAA190650.14755132139115704No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAAA189960.14701730402026852No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAA187190.1448734951545276No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAA186610.14442461098769377No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAA186290.14417695075771647No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAAC183700.1421724507713378No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAC183420.14195574807010766No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAC181750.14066327124491368No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAT180810.1399357693193554No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAT179110.13862007434760104No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAAT169170.13092712845393148No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAT165600.12816416901324734No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAC164820.1275604972026777No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAG161890.12529285822194816No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAC161690.12513807057821233No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAA158450.12263051074969228No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAT156370.1210207192548399No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAG152500.11802557834855204No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAC151820.1174993003598503No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAAT148950.1152780976722415No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAG148460.11489886794508877No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAC148160.11466668647948507No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAC147620.11424875984139839No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAA147170.11390048764299283No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAG146880.11367604555957592No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAA146050.11303367683807232No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTTACCCCATATAAGAAA145910.11292532548745723No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAAG144970.11219782356189896No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAC144210.1116096305157029No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAC143260.11087438920795781No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAAA143060.11071960156422202No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAA142730.11046420195205793No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAA142060.10994566334554298No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAA141810.10975217879087323No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAT140950.10908659192280926No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAT140420.10867640466690938No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAAG139090.10764706683606626No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAA137410.1063468506286855No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAC137290.106253978042444No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAG133850.10359163057018815No Hit
GCATAGCTCTTAAACACGAGAAGATGTCTCGTGCCCCCCATATAAGAAAC133210.10309631011023357No Hit
GCATAGCTCTTAAACACGAGAAGATGTCTCGTGCCCCCCATATAAGAAAA131120.1014787792331944No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAA131010.1013936460291397No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAATA130920.10132399158945858No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAG130860.10127755529633785No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAC130030.10063518657483425No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAACA129940.10056553213515314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT2500.044.0098158
CGTAGAC207.850851E-444.0098159
CGTAAGC408.307325E-944.0098159
GAACCGC254.439633E-544.00964412
GGACCCT254.439633E-544.00964412
ACCTATG351.4453508E-744.00879314
ACCTCCT2250.044.0087914
ACATGCG551.8189894E-1244.0087914
GAGGGTA650.044.0081147
CCCTCCT551.8189894E-1244.00691615
CAGCCGT254.4412573E-544.00691615
TTCCGAC502.7284841E-1144.0067516
CACGTTA207.8535464E-444.0067516
CCTCCTA351.4458783E-744.00674416
CTTTGTA351.4458783E-744.00674416
GGGCTGA254.4415618E-544.006416
TATCTTA502.7284841E-1144.006416
GCTTTTA3750.044.0064056
CGGCTAA302.5273403E-644.0060717
ACGTTAG207.854146E-444.00606517
Produced by FastQC (version 0.11.5)