FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_3d_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_3d_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13882330
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2454091.767779616245976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG693620.4996423511038853No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT465410.33525352012234255No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169610.1221768968177532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT302400.038.938941
AGCAGTG335700.035.065282
ATCAACG378100.030.91541311
TCAACGC381200.030.66999412
CAACGCA382350.030.58903913
GTATCAA384300.030.4057469
TGGTATC385000.030.400377
TATCAAC386900.030.2637610
GGTATCA387850.030.2008728
AACGCAG391150.029.95657314
GTGGTAT393150.029.8191436
CAGTGGT394100.029.773144
AGTGGTA394500.029.7553945
ACGCAGA393300.029.73450515
AGAGTAC394050.029.48540519
CGCAGAG404050.028.97565516
CAGAGTA404300.028.85340118
GAGTACT405100.028.58848820
GCAGAGT409200.028.486917
GTACTTT407500.028.33230222
Produced by FastQC (version 0.11.5)