FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_3c_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_3c_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12824655
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2270741.7706051351868726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG529100.4125647044696329No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT426550.3326015397685162No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT154010.12008900044484627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT276300.038.9947431
AGCAGTG305550.035.315382
ATCAACG348800.030.69784211
TCAACGC352100.030.38489712
CAACGCA354000.030.26532413
TGGTATC354750.030.2136277
GTATCAA356500.030.0907089
TATCAAC358600.029.93290310
CAGTGGT360050.029.9072234
GGTATCA359100.029.8422058
ACGCAGA363000.029.60402515
AACGCAG363150.029.59924114
AGTGGTA363900.029.5432455
GTGGTAT363750.029.5199416
AGAGTAC363350.029.40982619
CGCAGAG374400.028.76127616
CAGAGTA373850.028.69165218
GCAGAGT374600.028.6343217
GAGTACT373700.028.49498420
GTACTTT372900.028.40628622
Produced by FastQC (version 0.11.5)