FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_3b_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_3b_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22435979
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3730361.662668698343852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980140.43686081182372294No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT711270.31702204748899077No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255810.11401775692516025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT467500.038.5403021
AGCAGTG518150.034.7661442
ATCAACG577550.031.02131311
TCAACGC582300.030.74215512
CAACGCA585300.030.60337813
TGGTATC588300.030.4196437
GTATCAA589650.030.3928789
TATCAAC590950.030.3669810
GGTATCA594400.030.1533648
AACGCAG596750.030.1002614
CAGTGGT599800.029.9321864
ACGCAGA599200.029.92777415
GTGGTAT601900.029.7858816
AGAGTAC602650.029.62210319
AGTGGTA606250.029.5932125
CGCAGAG617850.029.06693616
CAGAGTA620650.028.91743318
GCAGAGT625150.028.7168317
GTACTTT619500.028.58668522
GAGTACT621900.028.57365820
Produced by FastQC (version 0.11.5)