FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_3a_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_3a_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13083501
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2319301.7726906582572965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG533090.40745210322527586No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT432030.3302097810058638No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157640.12048762789103619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT283750.038.8141981
AGCAGTG315200.034.9019242
ATCAACG351050.031.24804111
TCAACGC352550.031.10212912
CAACGCA353950.031.00995613
TGGTATC357400.030.65457
GTATCAA358150.030.6410989
TATCAAC360500.030.50850310
GGTATCA361550.030.328498
AACGCAG364250.030.2721414
ACGCAGA364900.030.1499215
CAGTGGT366750.030.0137674
GTGGTAT367500.029.9249866
AGTGGTA368250.029.9054055
AGAGTAC365250.029.8776619
CGCAGAG374700.029.41986816
CAGAGTA377250.029.13307418
GCAGAGT377500.029.09091217
GAGTACT375250.028.87535920
GTACTTT374100.028.81489222
Produced by FastQC (version 0.11.5)