FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_3HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_3HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4993975
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG558461.1182675123523846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA2609450.043.9291881
CGATAAT2611200.043.8678442
AGGACCA1013800.043.7584341
GGACCAT1011550.043.7540172
TAATGCG2605500.043.748335
ATAATGC2610850.043.7382354
GATAATG2623750.043.6724133
CATCCAA1018550.043.635696
GCGACCC2579350.043.629819
TGCGACC2584650.043.620358
CCATCCA1018250.043.5893255
ACCATCC1017300.043.571624
GACCATC1017600.043.568713
CGACCCA2587550.043.55617510
ATCCAAC1022000.043.546957
CATGTTA379750.043.5283552
TCCAACC1017250.043.511518
ATGCGAC2603100.043.494147
GACCCAT2598750.043.46624411
AATGCGA2613950.043.4630056