FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_3GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_3GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11017302
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAA3873493.5158244731786423No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAC3812973.4608926940552234No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAT3409543.0947141142178003No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAC3190622.896008478300767No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAA3131272.8421386651650287No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAG2969902.695669048556534No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAT2810152.5506698463925197No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAG2427932.2037428038189386No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAC1809821.6427070801907764No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAA1714991.5566333753944477No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAT1588291.441632443224303No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAG1381661.254081988494098No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAC1109201.0067800628502332No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAC1067090.9685583639261227No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAA954030.8659379583132059No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAA949380.8617173242596055No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAT944870.8576237630592317No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAT913140.8288236085386422No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAC911070.8269447456373621No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAA862560.7829140019943177No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAG848680.7703156362601297No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAG808630.7339637236049261No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAC793420.7201581657650848No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAG773990.7025222690636964No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAT772720.701369536752283No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAA693060.6290650832663024No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAC688010.6244813839177685No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAT651040.590925074033552No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAA639790.580713862613551No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAT618500.5613897122907224No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAG609000.5527669115360548No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG606030.5500711517211746No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAA592400.53769970179632No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAC589380.534958558819573No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAA575130.5220243576875717No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAG559320.5076742019053304No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAA558680.5070932974334369No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAC551320.5004128960066629No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAC546930.4964282543947693No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAT510680.4635254620414327No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAT506490.45972235307700554No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAG486440.4415237051684705No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAT466810.4237062758196154No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAA465010.42207248199241526No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAG444950.4038647574515068No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAG436260.39597716391907933No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAC435280.3950876539464925No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAA431680.3918200662920922No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAC426800.3873906696939051No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAT411740.37372126133966377No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAC400440.36346466675779604No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAA399450.3625660801528359No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAA398000.3612499684587025No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAT397500.36079613684003575No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAC373960.339429744233207No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCCATATAAGAAA371100.3368338273744334No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAC370090.33591708750472665No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAT366850.33297625861576635No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAA363190.329654211167126No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAG360790.3274758193975258No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAA360380.3271036774702191No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAC353810.3211403300009385No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAC352420.3198786781010451No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAA341400.30987622922563074No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAT337890.30669033126259043No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAG335640.30464808897859025No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAT334190.30333197728445677No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAG325430.295380847325416No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAG325240.2952083913103226No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAT317710.28837368713320194No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAT312270.2834359991221081No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCCATATAAGAAA300660.27289802893666704No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAG299430.271781603154747No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAG299420.27177252652237366No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAC285370.25901985803783906No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAA281700.25568873395682534No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAG273200.24797359643949127No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAT251240.22804131174764927No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAC249350.22632582822908914No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAG233960.21235689100652771No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAA220530.20016697372913986No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAC213020.19335042281676584No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAC208740.18946562416097879No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAT202630.18391980178087158No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAA191510.17382658658172392No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAA188350.1709583707517503No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAC185680.16853490990807005No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAC181710.16493148685585637No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAA181600.1648316438997497No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAG181360.16461380472278966No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAG181310.164568421560923No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAC177170.1608106957583626No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAT176810.1604839369929226No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAC176700.16038409403681592No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAA174140.15806047614924235No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAT171580.1557368582616688No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAT169220.1535947730215619No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAA167950.15244204071014844No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAC163880.14874785133420143No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAT160950.1460883980488145No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAG159360.14464521350145434No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAT159220.14451814064822766No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAG157880.14330187191020088No Hit
GCATAGCTCTTAAACTGGAGGAGACAATACCCTCACCCCATATAAGAAAC151610.1376108234121203No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAA150000.13614948560001353No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAT150000.13614948560001353No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAA147000.13342649588801322No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAG145590.13214669072337312No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAC143750.1304765903666796No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAC143080.12986845599766622No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAA142820.12963246355595953No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAG142100.12897894602507948No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAACC140690.12769914086043932No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAA140390.1274268418892393No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCCATATAAGAAA140200.12725438587414595No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAA137790.1250669174721724No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAC137570.12486723155995905No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAC135950.12339681711547891No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAC134050.1216722569645454No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAT133270.12096427963942534No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAG131530.11938494560646519No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAACA130690.11862250848710511No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAATA129490.11753331260230498No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAT129290.11735177995483831No Hit
GCATAGCTCTTAAACTGGAGGAGACAATACCCTCACCCCATATAAGAAAA128950.11704317445414496No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAA128900.11699779129227827No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAG128630.11675272221819824No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAAC128590.11671641568870492No Hit
GCATAGCTCTTAAACTGGAGGAGACAATACCCTCACCCCATATAAGAAAT128070.11624443080529154No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAC127160.11541845725931811No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAACG125500.11391173628534464No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAT125320.11374835690262461No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAAC125170.11361220741702459No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAATG124950.11341252150481125No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAA124850.11332175518107791No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAACT124580.11307668610699788No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAC123950.11250485826747783No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAC122640.11131581942657104No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAA122470.11116151667622436No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAC122450.1111433634114777No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAA122340.11104352045537103No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAAC121290.11009047405617092No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAG120850.1096911022317442No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAG120820.10966387233462421No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAT119210.10820253452251741No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAGC119170.10816622799302406No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAG118740.10777593280097068No Hit
GCATAGCTCTTAAACACGAGAAGATGTCTCGTGCCCCCCATATAAGAAAC118540.107594400153504No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAT117560.10670489018091726No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAT117480.10663227712193059No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAT116960.1061602922385172No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAA116340.10559754103137046No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAGA115900.10519816920694378No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAC115810.10511647951558377No Hit
GCATAGCTCTTAAACTGGAGGAGACAATACCCTCACCCCATATAAGAAAG114530.10395467057179698No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAAC114050.10351899221787693No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAAA112300.10193058155254343No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAAA112280.10191242828779677No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAG111190.10092307535910335No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAAA111140.10087769219723668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC254.4428063E-544.00424618
TCATCAG207.855693E-444.00424618
CGACGCC207.856923E-444.0028519
GTGCTCC207.8586803E-444.0008521
TCCCTCG207.859032E-444.0004543
GCCAATA650.043.9962523
ACTTAAT207.8636035E-443.9952628
CTCGAGG207.8637793E-443.99505625
TCTCACG207.8637793E-443.99505639
TTCATAG254.4482887E-543.99505625
CTTCCGG254.4497196E-543.99265732
GCATAGC10388200.043.9832421
CATAGCT10381900.043.975192
ATTCTGT139000.043.937343
ATAGCTC10409350.043.936613
TAGCTCT10405800.043.93054
AGCTCTT10413800.043.908945
TTCTGTC138750.043.9083024
GCTCTTA10423750.043.8795976
CCTGCAT185850.043.87462215
Produced by FastQC (version 0.11.5)