FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_2HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_2HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4718242
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288790.6120711909223817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA2555950.043.938981
CGATAAT2562450.043.8128172
TAATGCG2552200.043.7514155
ATAATGC2556400.043.750844
GATAATG2568700.043.7033963
AGGACCA942350.043.6880341
GGACCAT941900.043.6383442
CATCCAA948800.043.6237686
TGCGACC2538600.043.597368
CCATCCA946950.043.5872465
GCGACCC2534050.043.579729
AATGCGA2557700.043.572246
ATCCAAC952400.043.562377
GACCATC947100.043.5566753
GGCTGAG466500.043.5358623
TCCAACC948900.043.5203828
CCGTCCC392600.043.51469
ACCATCC948250.043.4862064
CGACCCA2542250.043.47204610
ATGCGAC2557200.043.4621477
Produced by FastQC (version 0.11.5)