FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_2GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_2GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12153683
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAA4556263.7488718440327924No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAC4555573.7483041148925804No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAT4022003.3092849303375775No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAC3693113.0386756014617133No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAA3675183.0239228717747535No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAG3456962.844372360213772No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAT3270402.6908715654341155No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAG2812682.314261446509671No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAA1740751.4322818852523964No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAC1699981.3987364982285615No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAT1536661.2643574791279317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1406491.1572541426331426No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAG1377891.1337221811692801No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAC1201030.9882025061868077No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAC1197270.9851087937705796No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAA1108670.9122090809839288No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAA1071980.8820207010500439No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAC1051500.8651698419318654No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAT1022830.8415802847581264No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAG996370.8198091064247768No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAA991040.8154236045156024No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAT973830.801263287844516No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAC931500.7664343392862888No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAG925700.7616621233250859No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAC923580.7599177961116808No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAT917150.7546272187615886No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAA854230.7028569035410912No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAA824220.6781647999211432No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAT821010.6755236252253741No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAG815490.6709817921036776No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAT773540.6364655059704947No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAC767350.6313723996256937No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAG735420.6051005279634165No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAA722390.5943794979678176No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAG692470.5697614459748539No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAT682680.5617062745506856No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAG596430.4907401320241774No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAC588880.4845280233160598No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAA569290.4684094525091695No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAA546290.4494851478354339No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAC540260.4445236888274937No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAA533900.4392907071872781No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAC533730.4391508318918636No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAT517770.42601901004000187No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAA488700.402100334524111No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAC461930.38007408947559357No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAT461300.37955572808670424No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAG460760.3791114183247991No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAG458100.37692278134948887No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAT451330.3713524534085676No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAT444680.3658808609702919No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAC438010.3603928126149086No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCCATATAAGAAA434020.35710985715194316No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAA430940.35457564591737334No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAG418450.3442989256836796No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAT397820.32732464718719423No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAC390640.3214169729455672No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAA383050.3151719524032345No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAC382180.31445612000905404No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAG376050.3094123814155758No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAG363300.2989217342594833No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCCATATAAGAAA358230.2947501592727077No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAC353860.29115454138469793No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAA352710.2902083261510112No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAA351930.28956654538381493No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAT350890.28871083769421996No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAC346260.28490129288381144No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAT337610.2777841087347761No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAA336240.27665687841290576No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAT320860.26400227815716437No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAT316310.26025855701518624No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAA315820.25985538704605016No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAG314870.25907373098343933No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAC311940.25666293912717647No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAG300940.24761218471799867No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAG300140.2469539480336948No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAC283250.23305692603632988No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAC281080.23127145903015572No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAG280430.23073664172415884No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAT275420.22661443448870602No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAA269120.22143082059981326No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAC261040.21478263008834442No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAA251240.20671923070562231No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAC250760.20632428869504002No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAG248770.2046869249428342No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAT247980.20403691621708417No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAT239640.19717479878321656No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAA237880.19572667807774813No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAG230940.19001647484141226No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAT227740.1873835281041969No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAA223400.18381259409184855No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAG220240.18121255918884835No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAA209700.1725402908731452No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAG207070.1703763377734963No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAT205240.16887062135815126No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAG202550.1666573005071796No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAC201160.16551361426820166No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAA192820.15865149683433408No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAC191990.15796857627436883No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAC190430.15668501473997634No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCCATATAAGAAA190270.15655336740311557No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAA181050.14896718961651376No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAA179550.14773299583344407No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAC179400.1476095764551371No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAA178770.1470912150662478No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAC178450.1468279203925263No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAC178300.1467045010142193No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAT171650.14123290857594362No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAG171440.14106012144631386No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAT165900.1365018324075097No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAT164650.13547333758828498No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAC164060.1349878880336109No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAA163480.1345106664374906No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAC163200.13428028359798425No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAC159330.1310960636376644No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAC158990.13081631304683528No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAC158180.1301498484039776No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAA157950.12996060535724027No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAA157770.1298125021032719No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAT156730.1289567944136769No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAG154840.12740171024700908No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAC154540.12715487149039514No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAA153710.1264719509304299No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAC152700.1256409271164963No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAG152140.12518016143748362No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAT150350.12370735685635374No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAG147010.12095921869938521No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAA146720.12072060790132505No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAG146140.12024338630520477No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAA145150.11942881840837877No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAT144710.11906678823201165No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAA143480.11805474932989447No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAT143400.1179889256614641No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAAG142480.11723195347451468No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAA142190.11699334267645453No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAT141000.11601421560855256No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAT140170.11533129504858733No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAT138880.11426988839514737No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAAAA137710.11330721724435301No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAG135450.11144769861119465No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAAC134450.11062490275581484No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAT133090.10950590039249831No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAT132080.1086748765785647No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAG131350.10807423560413745No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAG129850.10684004182106774No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAG128920.10607484167556452No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAG127480.1048900156438176No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAT125350.10313746047185861No Hit
GCATAGCTCTTAAACCATTCCTTGATTACAGCTGCCCCATATAAGAAACA125320.10311277659619722No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAG124350.10231466461647881No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAG123980.10201023014998828No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAAC122760.10100641920642492No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTTACCCCATATAAGAAA121850.1002576749780293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTG1700.044.00465417
TTTCGGA1050.044.0037518
TCATGGG302.5282461E-644.00374618
GTTATCT207.8587135E-444.0008520
TCTCTAG207.859988E-443.999443
CGAGATT254.4457225E-543.99939743
CGCTACA408.3255145E-943.99885642
GGTCGAC351.4485704E-743.99631523
CTTTTTC207.8633346E-443.99559830
CGGCTTC650.043.9952329
TACGTGC1200.043.99522829
CTCCAGT207.863813E-443.99505626
GCCAGTC408.3346094E-943.9936071
GCATAGC11368800.043.971741
CATAGCT11381600.043.921462
ATAGCTC11381250.043.9127663
TAGCTCT11388000.043.89854
AGCTCTT11392950.043.873275
GCTCTTA11400250.043.863416
CTCTTAA11411850.043.8412487
Produced by FastQC (version 0.11.5)