FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_1HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_1HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5063068
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388670.7676570806475441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA3279800.043.9384421
CGATAAT3281250.043.8744242
ATAATGC3276300.043.7724724
TAATGCG3272050.043.7622345
GATAATG3290150.043.745913
AGGACCA816150.043.735371
GGACCAT814400.043.6770062
TGCGACC3253150.043.603738
CATCCAA820050.043.5997436
GCGACCC3246300.043.585939
AATGCGA3279150.043.573446
CATGTTA265900.043.5599632
CCATCCA819650.043.554015
ATGCGAC3272050.043.5404057
ACCATCC819300.043.5328644
CGACCCA3252850.043.52208710
ATCCAAC823750.043.4822277
TCCAACC821550.043.4758958
GACCCAT3263750.043.46867411
GACCATC819900.043.465373