FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n02_1GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n02_1GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15434326
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAC5606303.632358160634938No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAA5516993.5744936319214715No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAA5139543.329941326883986No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAC4981663.227649850080917No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAT4925833.1914772306869765No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAT4508532.9211058519821336No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAAG4256242.75764552336137No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAAG3915912.537143507270742No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAC2547361.6504510789781168No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAA2376731.5398987944144757No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAT2252211.4592214781520099No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAAG1921121.2447061180384553No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAC1639571.062288045490292No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAA1583371.0258757006946724No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAC1495320.9688275341598978No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAT1442170.9343913041619051No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAC1415130.916871912644582No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAA1380080.8941627901341463No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAA1374440.890508597524764No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAAG1319590.8549709264920282No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAT1245040.8066694975860949No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAC1236290.8010003157896237No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAT1229100.7963418681191522No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAAG1224710.7934975586235512No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAA1159880.7514937808103833No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAAG1146580.7428766244797473No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAC1142200.7400387940490566No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAA1111620.720225813553504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1028940.6666569048755352No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAT1019980.6608516627159489No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAC1013240.6564847729664386No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAA1002910.6497918989141477No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAT996960.6459368552925473No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAT968620.6275751853368914No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAAG931350.6034277104163797No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAC919580.5958018510170123No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAAG900560.5834786695577119No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAA874700.5667238076997985No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAC862730.5589683670022261No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAA843220.5463277113623232No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAAG833910.540295701930878No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAG798250.5171913564609171No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAAT778970.50469971931395No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAT739790.47931474299558013No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAC723280.46861780682875304No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAC717080.4646007865843964No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAA680030.44059585109191035No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAC675300.43753125338936083No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAA660240.42777378163452034No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAA654650.42415198434968915No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAAG653670.4235170359884844No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAT634260.41094117099768396No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAT627510.40656780218326344No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAT621400.4026090935230991No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAC590210.38240089006802114No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAA585270.3792002320023563No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAA579430.37541645809476876No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAAG574360.37213157218527065No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAAG560030.3628470721688786No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAC546500.3540808973453068No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAAG541560.350880239279642No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCCATATAAGAAA536930.347880432226195No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAT517400.3352268184564716No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAC492970.31939846288072443No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCCATATAAGAAA487240.31568595868714966No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAT486940.31549158673984207No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAAG460040.29806290213126246No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAA452140.2929444408521629No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAAG449730.29138298620879205No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAT443740.2875020263275507No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAA424930.2753149052313655No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAC414870.2687969659316513No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAA408310.2645466993505256No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAC401900.2603936187430536No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAC401040.2598364191607719No Hit
GCATAGCTCTTAAACAATAAACACTATACCATGTACCCCATATAAGAAAG395870.2564867426021713No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAA393470.2549317670237106No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAT390110.2527548012138658No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAT366260.23730223140291323No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAT354280.22954031164043057No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAC336820.21822786430712943No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAA335080.21710050701274547No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAAG334210.21653682836555352No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAAG331330.21467085767140076No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAC326160.21132118111280013No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAAG321120.2080557323980328No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAA307880.1994774504568583No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAT296480.1920913164591703No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAG294380.19073071282801723No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAA290010.18789936146223685No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAC286470.1856057724840074No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCCATATAAGAAA277200.1795996793122032No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAAT269040.17431276234543705No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAC260600.1688444315611838No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAC259620.16820948319997905No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAAG257710.1669719818021208No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAC254200.16469783001862212No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAT252890.16384907251537903No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAAG244460.15838722079603607No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAA239600.15523839524965327No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAT239370.15508937675671747No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAA221850.14373805503395481No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAC215870.13986357421762377No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAT215470.13960441162121365No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAA214510.1389824213898294No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAT206950.1340842483176784No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAA205380.13306703512676873No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAAG201990.1308706321221931No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAC199320.12914072179115563No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAA198540.12863535472815593No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAC198380.1285316896895919No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAC197380.12788378319856664No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAAG195440.12662684460597762No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAC190990.12374366072091518No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAA189710.12291434041240286No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAAC189470.1227588428545568No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAT186600.12089935122531427No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAAG185610.12025792379919927No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAC183750.11905281772589228No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAT181780.11777644193857251No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAA180740.11710261918790622No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAAT180060.11666204277400904No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAAA179040.11600117815316328No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAAG177490.11499692309207411No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAT177380.11492565337806135No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAA177060.11471832330093325No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAT175620.11378533795385688No Hit
GCATAGCTCTTAAACTCTGCGTGAGTATTTTATACCCCCATATAAGAAAT172590.11182218128605033No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAAA170690.11059115895310231No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAAA170390.11039678700579475No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAAC168930.10945084352889786No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAAG168470.10915280654302624No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAAC166040.10757839376983486No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTTACCCCATATAAGAAA165950.10752008218564257No Hit
GCATAGCTCTTAAACCACATGACAGGACTTCAGCTCCCCATATAAGAAAC163430.10588735782825891No Hit
GCATAGCTCTTAAACCTGTCACGGACTCTGCACCCCCCCATATAAGAAAA160320.10387236864117033No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAAT157760.10221372802414566No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAAC157550.10207766766103035No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAAA154410.10004324127921102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAC254.4388282E-544.0110669
GGTATTC351.4448597E-744.0107810
GACCAGC302.5255104E-644.01077710
TCGTAGG302.5255104E-644.0107778
AGACCGC207.850805E-444.00992212
ACATTTG207.851432E-444.0092114
CTCCTTA408.309144E-944.008787
CCAAGTC254.4414628E-544.0066415
CCTTGTG254.4414628E-544.0066415
CACTTCG254.4414628E-544.0066415
CCATTGG254.4414628E-544.0066415
CCAACGG408.312782E-944.00663815
TAGCGAC551.8189894E-1244.00564216
CCGCACT12450.044.00564216
CATTCGT302.5275403E-644.0056416
GCTTTCT800.044.0056416
TCAAAAG207.8548177E-444.00535617
TATGCCT207.8548177E-444.00535617
CCGCTAA207.8548177E-444.00535617
CTGCGGT302.5276531E-644.00535617
Produced by FastQC (version 0.11.5)