FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26070794
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGC1120750.42988717566484547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG349810.13417696446069116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC119750.019.72247321
AGCAAGT112150.017.627623
AAGTCGA93850.017.1913096
GGGAATG32850.017.1031721
CAAGTCG94650.017.0704785
CGAAAGC115600.017.07032810
AACTCCC47750.017.0522421
GACTAAC40900.016.9903771
CCGGGAT35350.016.6404931
GCGCGAT32800.016.614081
GGGACCT23550.016.5924781
GACTGCG52550.016.5798911
CGCTATC40000.016.5013871
GTAACTG37300.016.3934421
GAACCTA40950.016.273311
TTGAAAA40600.016.2614718
TCCCGAT33050.016.232771
GTCCGCT44600.016.1682478
GACCTGG33700.016.0450291
CCTACCA40250.016.0315591
Produced by FastQC (version 0.11.5)