FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15751144
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA43050.018.1700338
ACTGAAC42600.018.169581
CAATGGA58650.018.0095378
TGACTAG46300.017.9651641
CTGCCTA51800.017.578281
CCGGGAT50400.017.3789081
TATGCCC52000.017.3692911
GGGACCT38600.017.3588281
TGCTACC57300.017.320651
ACTGATG43800.017.3121131
TTTATGC50850.017.2870751
CGATTGA55600.017.2457851
ATGCGAT45200.017.1941321
CGGACTG39100.017.1368481
GAATGAA42700.017.1186051
ACTGCTC62050.017.0610491
CTGATCC40000.017.0400831
GGTATTG55750.017.029841
GACCTGG46800.016.8980311
GGAATAA54850.016.888031
Produced by FastQC (version 0.11.5)